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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital purpose in promoting cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually explained. Two supplemental splice variants were being noted but comprehensive nucleotide sequence information and facts is just not however readily available.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This manual outlines further doable optimization of cellular and immunoassay parameters to ensure the best possible final results are...
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This tutorial outlines further more possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which add for the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates at the G1-S period boundary and is particularly degraded as cells development by means of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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